Fakultät für Mathematik und Naturwissenschaften


Next to the standard techniques to quantify and identify nucleic acids (PCR, qPCR, Sanger Sequencing) we use next-generation sequencing (NGS) for DNA, Chromatin, RNA, sRNA analysis using Illumina, BGI and Oxford Nanopore platforms. We analyze transcriptomics using differential enrichment for poly-A RNAs, ribo-depletions or Dual-Seq of pathogens and host in infected cells. We also characterize epigenetic states by ChIP seq and MNAse Seq allowing for integrative analysis of the epigenome and transcription. Application of different protocols and constantly improvements for sRNA Seq, range from 2´-O me analysis to downscaling to minimal input and single cell inputs.

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